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Plasmid DNA Preparation> | … The genes of P450cam (Uniport: P00183) and Pdx (Uniport: P00259) were synthesized by GenScript and inserted into pET-20b plasmid with a C-terminal histidine tag … | Get A Quote |
Protein-protein interactions occur in a wide range of biological processes and are of great significance to life function. Characterization of transient protein-protein interactions remains a significant barrier to our understanding of cellular processes. Nanopores provide unique nanoscale environments that accommodate single molecules from the surrounding bulk solution. This method permits label-free sensing at the single-molecule level with extremely high sensitivity. Herein, the interaction between a single P450cam monooxygenase and its redox partner putidaredoxin (Pdx) was monitored transient ionic current by using functionalized glass nanopores. Results show that the volume of P450cam determines the block... More