Add 200 µl of ddH2O to make the final concentration of 1 µg/µl in PBS Buffer. 0.04-0.2 µl of Multiple Tag is recommended for each lane of the minigel (for accurate loading, dilute with PBS Buffer).
描述
Multiple Tag is a specially designed fusion protein that can serve as an excellent positive control for western and dot blots. The fusion protein has an apparent molecular weight of ~40 kDa on SDS-PAGE and it contains 16 commonly used tags from N terminus to C terminus in the order as shown in the table below. TagSequenceTrxMSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLAT7CMASMTGGQQMGHSVQPELAPEDPEDCc-MycEQKLISEEDLCVSV-gYTDIEMNRLGKGlu-GluCEEEEYMPMEV5GKPIPNPLLGLDSTE-tagGAPVPYPDPLEPRFlag-tagDYKDDDDKS-tagKETAAAKFERQHMDSHAYPYDVPDYACKT3KPPTPPPEPETE2GVSSTSSDFRDRAU1DTYRYIAU5TDFYLK6x HisHHHHHH