GenScript Anti-DYKDDDDK IP Resin is designed for quick and efficient immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex. The resin is an agarose beads coupled to Mouse anti-DYKDDDDK monoclonal antibody (clone 6H8E4). The antibody recognizes N-terminal, internal and C-terminal fusion protein containing DYKDDDDK tag. It is supplied as a suspension in 50 % glycerol containing 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.4,0.02 % (w/v) sodium azide (PBS/A).
GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein. It is composed of agarose and conjugated THETM anti-His monoclonal antibody. His-tagged protein in the crude samples can bind to the anti-His monoclonal antibodies coupled with the agarose, and the nonspecific proteins can be eliminated by washing. As a result, the His-tagged protein will finally be eluted with high recovery. Moreover, this product can be used for immunoprecipitation of His-tagged protein.
DYKDDDDK (Flag) Tag Antibody Plate is a microtiter plate coated with THETM Anti DYKDDDDK (Flag) Tag Antibody. The plate is designed for the rapid capturing of DYKDDDDK (Flag) tagged protein expressed in different systems, including, E. coli, yeast and mammalian extracts and cell culture supernatants. It can be applied for the detection of DYKDDDDK (Flag) tag located at N-terminus, C-terminus, or internal of the protein. The plate demonstrates high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the DYKDDDDK (Flag) antibody plate than ones with higher M.W. The plate is tolerable with several common used reagents. Avoid using detection reagents that can recognize mouse IgG, such as anti mouse IgG antibodies and protein A.
His Tag Antibody Plate is a microtiter plate coated with THETM Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.