目录产品 » 稳定细胞系 » Human Recombinant D2 Dopamine Receptor Stable Cell Line
CHO-K1/D2/Gα15 Stable Cell Line

Figure 2. 1 μg of membranes prepared from CHO cells stably expressing D2 receptors were incubated with indicated concentrations of [3H]spiperone in the absence (total binding) or presence of 1000-fold excess unlabeled haloperidol (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

CHO-K1/D2/Gα15 Stable Cell Line

Figure 3. 1 μg of membranes prepared from CHO cells stably expressing D2 receptors were incubated with indicated concentrations of haloperidol in the presence of 1nM [3H]haloperidol. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

CHO-K1/D2/Gα15 Stable Cell Line

Figure 1: Dopamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/D2/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a D2 receptor agonist, dopamine. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of dopamine (Mean ± SD, n = 2). The EC50 of dopamine on D2 expressing CHO-K1/Gα15 cells was 18.8 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/D2/Gα15 Stable Cell Line

Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain, where it controls a variety of functions including locomotor activity, cognition, emotion, positive reinforcement, food intake, and endocrine regulation. It also plays multiple roles in the periphery as a modulator of cardiovascular function, catecholamine release, hormone secretion, vascular tone, renal function, and gastrointestinal motility. The dopamine receptor family consists of five members, which are classified into two groups, D1-like (D1 and D5) and D2-like (D2, D3, and D4). Dopamine receptor 2 is mainly expressed in the brain. It has splicing variants, D2L and D2S. D2R receptor is implicated in a number of neurological and psychiatric conditions. Drugs acting at dopamine D2 receptors (D2R) are commonly used to alleviate symptoms for Parkinson's disease, schizophrenia, and depression.
M00152
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Synonyms

D2 receptor, D2A and D2B, D2(415) and D2(444), dopamine D2 receptor, D(2) dopamine receptor, dopamine receptor 2, dopamine receptor D2, Drd-2, D2 receptor, D2R

Applications Functional assays for D2 receptor

Storage Liquid nitrogen immediately upon delivery
Species Human

Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

  • CHO-K1/D2/Gα15 Stable Cell Line
  • CHO-K1/D2/Gα15 Stable Cell Line

    Figure 2. 1 μg of membranes prepared from CHO cells stably expressing D2 receptors were incubated with indicated concentrations of [3H]spiperone in the absence (total binding) or presence of 1000-fold excess unlabeled haloperidol (nonspecific binding, NSB). Binding was terminated by rapid filtration. Specific binding was defined by subtracting NSB from total binding. Data were fit to one-site binding equation using a non-linear regression method.

  • CHO-K1/D2/Gα15 Stable Cell Line
  • CHO-K1/D2/Gα15 Stable Cell Line

    Figure 3. 1 μg of membranes prepared from CHO cells stably expressing D2 receptors were incubated with indicated concentrations of haloperidol in the presence of 1nM [3H]haloperidol. Binding was terminated by rapid filtration. Data were fit to one-site competition equation using a non-linear regression method.

  • CHO-K1/D2/Gα15 Stable Cell Line
  • CHO-K1/D2/Gα15 Stable Cell Line

    Figure 1: Dopamine-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/D2/Gα15 cells. The cells were loaded with Calcium-4 prior to stimulation with a D2 receptor agonist, dopamine. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of dopamine (Mean ± SD, n = 2). The EC50 of dopamine on D2 expressing CHO-K1/Gα15 cells was 18.8 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is the response and starts at Bottom and goes to Top with a sigmoid shape.


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