目录产品 » 稳定细胞系 » Human Recombinant CCKB Cholecystokinin Receptor Stable Cell Line
CHO-K1/CCKB Stable Cell Line

Figure 1: CCK8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CCKB cells.
The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to stimulation with CCKB agonist, CCK8. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of CCK8 (mean ± SD, n = 3). The EC50 of CCK8 on CHO-K1/CCKB cells was 0.945 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

CHO-K1/CCKB Stable Cell Line

Human Recombinant CHO-K1/CCKB Stable Cell Line stably overexpress the Cholecystokinin Receptor gene (CCKB). This stable cell line product is designed for measuring the In Vitro function of CCKB.
M00154
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产品简介
Product Description Human Recombinant CHO-K1/CCKB Stable Cell Line stably overexpress the Cholecystokinin Receptor gene (CCKB). This stable cell line product is designed for measuring the In Vitro function of CCKB.
Culture Properties Adherent
Stability Stable through more than 16 passages without significant changes in assay performance or expression profile. 
Size Two vials of frozen cells (1×106 per vial in 1 mL) 
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Gibco), 45% FBS (Cat. No. A5669701, Gibco), 10% DMSO (Cat. No. D2650, Sigma)

图例
  • CHO-K1/CCKB Stable Cell Line
    • CHO-K1/CCKB Stable Cell Line

    Figure 1: CCK8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CCKB cells.
    The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to stimulation with CCKB agonist, CCK8. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of CCK8 (mean ± SD, n = 3). The EC50 of CCK8 on CHO-K1/CCKB cells was 0.945 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response.
    Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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