目录产品 » 稳定细胞系 » Human Recombinant CCKB Cholecystokinin Receptor Stable Cell Line
CHO-K1/CCKB Stable Cell Line

Figure 1: CCK8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CCKB cells.
The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to stimulation with CCKB agonist, CCK8. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of CCK8 (mean ± SD, n = 3). The EC50 of CCK8 on CHO-K1/CCKB cells was 0.945 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

CHO-K1/CCKB Stable Cell Line

Human Recombinant CHO-K1/CCKB Stable Cell Line stably overexpress the Cholecystokinin Receptor gene (CCKB). This stable cell line product is designed for measuring the In Vitro function of CCKB.
M00154
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产品概述
Product Description Human Recombinant CHO-K1/CCKB Stable Cell Line stably overexpress the Cholecystokinin Receptor gene (CCKB). This stable cell line product is designed for measuring the In Vitro function of CCKB.
Applications Functional assay for CCKB receptor (Calcium Flux)

产品简介
Expressed Gene Codon optimized from NM_176875; no expressed tags
Target Protein NP_795344.1
Host Cell CHO-K1
Size Two vials of frozen cells (1×106 per vial in 1 mL) 
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages without significant changes in assay performance or expression profile. 

培养条件
Culture Properties Adherent
Freeze Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Gibco), 45% FBS (Cat. No. A5669701, Gibco), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)

技术背景
Gene Synonyms CCK-2R; CCK-B; CCK2R; GASR; CCKBR
Background Cholecystokinins (CCK) are derived from preprocholecystokinin. They are widely expressed in gastrointestinal tissues and in the CNS. There are two cholecystokinin receptors, referred to as A and B (CCKAR and CCKBR). CCKBR can be activated by the smaller CCK-4. In the CNS, CCKBR has been implicated in anxiety, depression, schizophrenia, depression, and opioid analgesia. In the stomach, CCK-2 mediates gastrin-stimulated gastric acid secretion.

图例
  • CHO-K1/CCKB Stable Cell Line
    • CHO-K1/CCKB Stable Cell Line

    Figure 1: CCK8-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/CCKB cells.
    The cells were loaded with Calcium-4 (Cat. No. R8141; Molecular Devices) prior to stimulation with CCKB agonist, CCK8. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of CCK8 (mean ± SD, n = 3). The EC50 of CCK8 on CHO-K1/CCKB cells was 0.945 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response.
    Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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