| Product Description |
Recombinant CHO-K1 cells stably overexpress human bombesin receptor 2 (BB2)
on the surface and contain high levels of G protein Gαq to couple with the
receptor in downstream signaling pathways. |
| Applications |
Calcium mobilization
assay, IP-One assay. |
| Expressed Gene |
Codon optimized from
NM_005314; no expressed tags |
| Target Protein |
NP_005305 |
| Host Cell |
CHO-K1 |
| Size |
Two vials of frozen
cells (>1×106 per vial in 1 mL) |
| Storage |
Store cells in liquid nitrogen immediately upon
receipt. Thaw and recover cells within one year from the date received. |
| Mycoplasma Status |
Negative. The
mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit
(Cat. No. LT07-318, Lonza). |
| Stability |
Stable through more than 16 passages with no significant
changes in assay performance or expression profile. |
| Culture Properties |
Adherent |
| Freeze Medium |
45% Ham’s F-12K (Kaighn’s) (Cat. No.21127, Life
Technologies), 45% FBS (Cat. No.10099-141, Gibco), 10% DMSO (Cat. No.D2650,
Sigma) |
| Complete Growth Medium |
Ham’s
F-12K (Kaighn’s), 10% FBS |
| Culture Medium |
Ham’s
F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. No.10131-035, Gibco) |
| Gene Synonyms |
GRPR, BB2R, BRS2 |
| Background |
Bombesin
is a peptide isolated from the skins of various frogs. There are two peptides,
neuromedin B (NMB) and gastrin-releasing peptide (GRP) that can be considered
as its mammalian counterparts. The bombesin receptor family has been classified
into three subtypes according to the affinity of each peptide to each receptor:
BB1, BB2, and BB3. BB2 receptor, which binds with GRP, is involved in a wide
range of physiological functions. In the gastrointestinal tract, BB2 receptor
activation causes stimulation of acid secretion, pancreatic and intestinal
secretion, and gastrointestinal motility. In CNS, it participates in regulating
circadian rhythms, controlling body temperature, affecting grooming behaviors,
and modulating fear, stress, anxiety, and memory. Additionally, BB2 receptors
are one of the most frequently and widely overexpressed and ectopically
expressed G protein-coupled receptors in human tumors. BB2 receptors may play a
potential role in the treatment and management of these tumors. |
Figure 1: Neuromedin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/BB2 cells. The cells were loaded with Calcium-4 prior to stimulation with a BB2 receptor agonist, neuromedin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neuromedin B (Mean ± SEM, n = 2). The EC50 of Neuromedin B on BB2 in CHO-K1 cells was 77.68 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.
For research use only. Not intended for human or animal clinical trials, therapeutic
or diagnostic use.
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