目录产品 » 稳定细胞系 » Human Recombinant NK2 Tachykinin Receptor Stable Cell Line
CHO-K1/NK2 Stable Cell Line

Figure 1: Neurokinin A-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK2 cells. The cells were loaded with Calcium-4 prior to stimulation with an NK2 receptor agonist, Neurokinin A. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neurokinin A (Mean ± SD, n = 2). The EC50 of Neurokinin A on this cells was 46.5 nM.
Note:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/NK2 Stable Cell Line

Tachykinins are peptides sharing a common C-terminal amino acid sequence: Phe-X-Gly-Leu-Met-NH2. This neuropeptide family is composed of substance P, neurokinin A, and neurokinin B, which are widely distributed in mammalian central and peripheral nervous systems. These three molecules serve as both neurotransmitters and neuromodulators. Their actions are mediated by binding with three distinct receptors, namely NK1, NK2, and NK3. In particular, NK2 is expressed in gastrointestinal tract. Activation of NK2 is chiefly responsible for the regulation of intestinal motor functions (both excitatory and inhibitory), secretions, inflammation, and visceral sensitivity. Antagonists of NK2 may be useful in the treatment of irritable bowel syndrome.
M00200
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Synonyms

Human Recombinant NK2 Tachykinin Receptor Stable Cell Line

Applications Functional assays for NK2 receptor

Storage Liquid nitrogen immediately upon delivery
Species Human

Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

  • CHO-K1/NK2 Stable Cell Line
  • CHO-K1/NK2 Stable Cell Line

    Figure 1: Neurokinin A-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK2 cells. The cells were loaded with Calcium-4 prior to stimulation with an NK2 receptor agonist, Neurokinin A. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neurokinin A (Mean ± SD, n = 2). The EC50 of Neurokinin A on this cells was 46.5 nM.
    Note:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is the response and starts at Bottom and goes to Top with a sigmoid shape.


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