目录产品 » 稳定细胞系 » Human Recombinant NK3 Tachykinin Receptor Stable Cell Line
CHO-K1/NK3 Stable Cell Line

Figure 1: Neurokinin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK3 cells. The cells were loaded with Calcium-4 prior to stimulation with an NK3 receptor agonist, Neurokinin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neurokinin B (Mean ± SEM, n = 2). The EC50 of Neurokinin B on NK3 in CHO-K1 cells was 12.0 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/NK3 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human tachykinin receptor 3 (NK3) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
M00201
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产品概述
Product Description Recombinant CHO-K1 cells stably overexpress human tachykinin receptor 3 (NK3) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Applications Calcium mobilization assay, IP-One assay.

产品简介
Expressed Gene Codon optimized from NM_001059; no expressed tags
Target Protein NP_001050
Host Cell CHO-K1
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件
Culture Properties Adherent
Freeze Medium 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. No. 10131-035, Gibco)

技术背景
Gene Synonyms TACR3, NK3R, NMKR
Background Tachykinins are peptides sharing a common C-terminal amino acid sequence: Phe-X-Gly-Leu-Met·NH2. This neuropeptide family is composed of substance P, neurokinin A, and neurokinin B, which are widely distributed in mammalian central and peripheral nervous systems. These three molecules serve as both neurotransmitters and neuromodulators. Their actions are mediated by binding with three distinct receptors, namely NK1, NK2, and NK3. NK3 receptors show affinity for neurokinin B. They are predominantly expressed in both the peripheral and central nervous systems. NK3 receptors appear to modulate monoaminergic and amino acid neurotransmission.  Studies show that manipulating modulation of NK3 receptor activity may have therapeutic utility in psychiatric diseases such as schizophrenia and affective disorders.

图例
  • CHO-K1/NK3 Stable Cell Line
    • CHO-K1/NK3 Stable Cell Line

    Figure 1: Neurokinin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK3 cells. The cells were loaded with Calcium-4 prior to stimulation with an NK3 receptor agonist, Neurokinin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neurokinin B (Mean ± SEM, n = 2). The EC50 of Neurokinin B on NK3 in CHO-K1 cells was 12.0 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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