目录产品 » 稳定细胞系 » Human Recombinant NK3 Tachykinin Receptor Stable Cell Line
CHO-K1/NK3 Stable Cell Line

Figure 1: Neurokinin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK3 cells. The cells were loaded with Calcium-4 prior to stimulation with an NK3 receptor agonist, Neurokinin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neurokinin B (Mean ± SEM, n = 2). The EC50 of Neurokinin B on NK3 in CHO-K1 cells was 12.0 nM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/NK3 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human tachykinin receptor 3 (NK3) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
M00201
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产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human tachykinin receptor 3 (NK3) on the surface and contain high levels of G protein Gαq to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. No. 10131-035, Gibco)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)

图例
  • CHO-K1/NK3 Stable Cell Line
    • CHO-K1/NK3 Stable Cell Line

    Figure 1: Neurokinin B-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NK3 cells. The cells were loaded with Calcium-4 prior to stimulation with an NK3 receptor agonist, Neurokinin B. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Neurokinin B (Mean ± SEM, n = 2). The EC50 of Neurokinin B on NK3 in CHO-K1 cells was 12.0 nM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is % stimulation of RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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