| 询价 | |
| M00327 | |
|
|
|
|
|
|
|
|
|
C5a1 receptor, C5aR1, CD88, C5R1, C5A, C5AR, complement component 5 receptor 1 (C5a ligand), C5a anaphylatoxin chemotactic receptor, C5a-R, complement C5a receptor, complement component 5, receptor 1, complement component 5a receptor 1, D7Msu1
| Storage | Liquid nitrogen immediately upon delivery |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen), 400 μg/ml G418 (Cat. #10131-035, Gibco) |
| Freeze Medium-DATA | 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma) |
Figure 1. C5a (65-74)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/C5a/Gα15 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist C5a (65-74). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of C5a (65-74) (Mean ± SD, n = 2). The EC50 of C5a (65-74) on this cell was 4.2 µM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
»
»
