CHO-K1/MT1/Gα15 Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human melatonin receptor 1A (MTNR1A) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
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| M00424 | |
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| 询价 | |
| M00424 | |
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| Product Description | Recombinant CHO-K1 cells stably overexpress human melatonin receptor 1A (MTNR1A) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways. |
| Culture Properties | Adherent. |
| Stability | Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL). |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies). |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 10% FBS. |
| Freeze Medium-DATA | 45% culture medium, 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma). |
Figure 1. Melatonin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MT1/Gα15 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with MT1 agonist, melatonin. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and normalized to plot against the log of the cumulative doses of melatonin (mean ± SEM, n = 3). The EC50 of melatonin on CHO-K1/MT1/Gα15 cells was 2.32 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is % stimulation of ∆RFU and starts at Bottom and goes to Top along a sigmoid curve. »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
