目录产品 » 稳定细胞系 » Human Recombinant Mas-related G Protein Coupled Receptor Member X2 Stable Cell Line
CHO-K1/MRGPRX2 Stable Cell Line

Figure 1. Cortistatin 14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MRGPRX2 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to stimulation with a MRGPRX2 receptor agonist, cortistatin 14. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (∆RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of cortistatin 14 (Mean ± SEM, n = 3). The EC50 of cortistatin 14 on this cell was 1.53 μM.

Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/MRGPRX2 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human mas-related G-protein coupled receptor member X2 (MRGPRX2) on the surface and contain high levels of G protein Gαq and Gαi to couple with the receptor in downstream signaling pathways.
M00425
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Product Description Recombinant CHO-K1 cells stably overexpress human mas-related G-protein coupled receptor member X2 (MRGPRX2) on the surface and contain high levels of G protein Gαq and Gαi to couple with the receptor in downstream signaling pathways.
Culture Properties Adherent
Stability Stable through more than 16 passages with no significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.

Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s), 10% FBS
Freeze Medium-DATA 45% Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma)

  • CHO-K1/MRGPRX2 Stable Cell Line
  • CHO-K1/MRGPRX2 Stable Cell Line

    Figure 1. Cortistatin 14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MRGPRX2 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular Devices) prior to stimulation with a MRGPRX2 receptor agonist, cortistatin 14. The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (∆RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of cortistatin 14 (Mean ± SEM, n = 3). The EC50 of cortistatin 14 on this cell was 1.53 μM.

    Notes:
    EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
    X is the logarithm of concentration. Y is the response
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.


For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.


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