Figure 1. LCA-induced concentration-dependent stimulation of intracellular cAMP accumulation in CHO-K1/GPBAR1 cells. The intracellular cAMP change was measured by Varioskan. The ratio of the luminescence signal were plotted against the log of the cumulative doses of LCA (Mean ± SD, n = 2). The EC50 of LCA on this cell was 6.06 μM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
CHO-K1/GPBAR1 Stable Cell Line
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