Figure 1: GLP-1 (7-37)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP1R/Gα15 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular DEVICES) prior to stimulation with GLP1R agonist, Glucagon-Like Peptide (GLP) I (7-37) (Cat. No. RP12738, GenScript). The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescence units (RFU) recorded and normalized to plot against the log of the cumulative doses of GLP-1 (7-37) (Mean ± SD, n = 2). The EC₅₀ of GLP-1 (7-37) on CHO-K1/GLP1R/Gα15 cells was 0.50 µM.
Notes:
EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC₅₀-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.

CHO-K1/GLP1R/Gα15 Stable Cell Line

GLP1R binds specifically the glucagon-like peptide-1 (GLP1) and has much lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP1R is known to be expressed in pancreatic beta cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a potential target for the treatment of diabetes. GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which over express GLP1R display improved memory and learning.

产品编号	M00451
规格	2 vials 大单询价
数量
价格	询价


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产品概述

Product Description

Recombinant CHO-K1 cells stably overexpress human glucagon-like peptide 1 receptor (GLP1R) and Gα15 subunit. Activated GLP1R stimulates the adenylyl cyclase pathway naturally. While the co-expression of GLP1R and Gα15 subunit makes activated GLP1R also triggers PLC signaling and then stimulates intracellular calcium mobilization. This cell line is recommended for screening and functional validating agonists of GLP1R.

Applications

Screening and functional validation of agonists of GLP1R

产品简介

Expressed Gene	Codon optimized from NM_002062; no expressed tags
Target Protein	NP_002053
Host Cell	CHO-K1/Gα15
Quantity	2 vials of frozen cells (> 1×10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability	Stable through more than 16 passages with no significant changes in assay performance or expression profile.

培养条件

Culture Properties	Adherent
Freeze Medium	45% Culture medium, 45% FBS (Cat. No. 10099-141, Gibco), 10% (V/V) DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	Ham's F-12K (Kaighn's) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium	Ham's F-12K (Kaighn's) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)

技术背景

Gene Synonyms

GLP-1R

Background

GLP1R binds specifically the glucagon-like peptide-1 (GLP-1) and has much a lower affinity for related peptides such as the gastric inhibitory polypeptide and glucagon. GLP1R is known to be expressed in pancreatic beta cells. Activated GLP1R stimulates the adenylyl cyclase pathway which results in increased insulin synthesis and release of insulin. Consequently, GLP1R has been suggested as a potential target for the treatment of diabetes. GLP1R is also expressed in the brain where it is involved in the control of appetite. Furthermore, mice which overexpress GLP1R display improved memory and learning.

图例

CHO-K1/GLP1R/Gα15 Stable Cell Line

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