Figure 1: GLP-1 (7-37)-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GLP1R/Gα15 cells. The cells were loaded with Calcium-4 (Cat. No. R8142, Molecular DEVICES) prior to stimulation with GLP1R agonist, Glucagon-Like Peptide (GLP) I (7-37) (Cat. No. RP12738, GenScript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescence units (RFU) recorded and normalized to plot against the log of the cumulative doses of GLP-1 (7-37) (Mean ± SD, n = 2). The EC50 of GLP-1 (7-37) on CHO-K1/GLP1R/Gα15 cells was 0.50 µM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is RFU and starts at Bottom and goes to Top along a sigmoid curve.
CHO-K1/GLP1R/Gα15 Stable Cell Line
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