Figure 1. Amylin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/AMY3 cells. The cells were loaded with Calcium-4 prior to stimulation with an AMY3 receptor agonist, Amylin. The intracellular calcium change was measured by FLIPR. The fluorescence signal were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Amylin (Mean ± SD, n = 4). The EC50 of Amylin on this cells was 0.1747 μM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom
CHO-K1/Ga15/AMY3 Stable Cell Line
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