MonoRab™ Anti-Humanized VHH Affinity Resin FF

GenScript’s MonoRab™ Anti- Humanized VHH Affinity Resin FF (Cat. No. L00951) is designed for purification of humanized VHH single domain antibody from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

价格	￥3800 在线订购包邮(港澳台除外)
产品编号	L00951-1
规格	1 ml Resin (Total 2 ml) 5 ml Resin (Total 10 ml) 10 ml Resin (Total 20 ml) 25 ml Resin (Total 50 ml) 大单询价
数量
参考货期


咨询产品问题

产品概述产品属性图例使用条款

产品概述

Product description

Camelid VHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx) is a peptide chain about 110 amino acids in length, containing one variable domain of a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelid VHH antibody is able to bind selectively to a specific antigen. This singledomain antibody has a broad range of applications in biotechnical and therapeutic research fields due to its small size, simple production and high affinity. However, their sequences may increase the risk of immunogenicity and anti-drug antibody (ADA) development in humans, and thus, sdAbs are routinely humanized during development.
GenScript’s MonoRab™ Anti- Humanized VHH Affinity Resin FF (Cat. No. L00951) is designed for purification of humanized VHH single domain antibody from commonly used protein expression systems such as bacteria, yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically bind to the Anti- Humanized VHH monoclonal antibody coupled to the resin. Nonspecific binding reactions are eliminated by stringent washing steps, and the purified target protein is eluted with a high recovery rate.

Key Features

● Can be recycled at least 5 times with no loss of binding capability
● Can be reused up to 10 times with minimum loss of binding capability
● Acid buffer elution (pH 2.5)
● Compatible with commonly used bacterial lysis reagents

产品属性

Formulation	50% settled resin in TBS with 0.02% sodium azide
Matrix	4% highly cross-linked agarose
Average Particle Size	90 μm (45-165 μm)
Ligand	MonoRab™ Anti-Humanized VHH Antibody
Binding Capacity	> 2 mg Humanized VHH per mL settled resin (depending on amino acid sequence of different Humanized VHHs)
Elution Method	Acid buffer elution (pH 2.5)
Reuse Capability	When stored at 2 - 8° C, the resin can be recycled at least 5 times with no loss of binding capability. While, it can be reused up to 10 times with minimum loss of binding capability.
Reagents Compatibility	Compatible with commonly used bacterial lysis reagents.
Application	purification of humanized VHH single domain antibody
Storage	Stored at 2°C~8°C. Do not freeze.

图例

MonoRab™ Anti-Humanized VHH Affinity Resin FF

Figure 3. Reuse times of anti-humanized VHH affinity resin FF

After VHH was captured by MonoRab™ Rabbit Anti-Humanized VHH Affinity Resin FF, the resin was washed twice with TBS (10 times bed volume), and eluted with 10 times bed volume of acid elution buffer (pH 2.5). The resin was washed with TBS and elution buffer again. Then the resin was regenerated by 10 times bed volume of regeneration buffer A and regeneration buffer B. Binding of VHH was repeated 9 times, and the binding capacity did not decrease significantly, indicating that the resin can be reused at least 10 times. »

Figure 1. Purification of Humanized VHH#1 protein from E. coli lysate

Lanes:
1. Protein marker
2. Whole cell lysate
3. Flow through
4. Eluate of target Humanized VHH single domain antibody-1 (M.W.=15 kDa) »

Figure 2. Purification of Humanized VHH#2 protein from E. coli lysate

Lanes:
1. Protein marker
2. Whole cell lysate
3. Flow through
4. Eluate of target Humanized VHH single domain antibody-2 (M.W.=15 kDa) »

使用条款

Terms of Use

This product may be the subject of one or more patents filed by GenScript Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer should not sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for any commercial purposes. For commercial use, please contact GenScript at product@genscript.com.

For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.