Products/Services Used | Details | Operation |
---|---|---|
PCR Cloning and Subcloning> | ... Plasmid encoding HA-tagged ß1AR and HA-ß2AR were generous gifts from Prof. Hitoshi Kurose (Kyushu University, Japan). The cDNA encod- ing rat GLUT4 was purchased from GenScript (Piscataway, NJ) and subcloned into pEGFP-C1 vector. ... | Get A Quote |
Insulin resistance is a condition in which cells are defective in response to the actions of insulin in tissue glucose uptake. Overstimulation of β-adrenergic receptors (βARs) leads to the development of heart failure and is associated with the pathogenesis of insulin resistance in the heart. However, the mechanisms by which sustained βAR stimulation affects insulin resistance in the heart are incompletely understood. In this study, we demonstrate that sustained βAR stimulation resulted in the inhibition of insulin-induced glucose uptake, and a reduction of insulin induced glucose transporter (GLUT)4 expression that were mediated by the β2AR subtype in cardiomyocytes and heart tissue. Overstimulation of β... More