Products/Services Used | Details | Operation |
---|---|---|
Gene Synthesis> | ...Human ROCK2, ROCK2T240A, ROCK2T240E, ROCK2D440–849 (D60 nm) and ROCK21–408 (kinase domain alone) were expressed in Sf9 insect cells from a synthetic gene (GenScript) using the Bac-to-Bac system (Invitrogen) with a N-terminal His10-StrepII-tag…. | Get A Quote |
AMP-activated protein kinase (AMPK) inhibits several anabolic pathways such as fatty acid and protein synthesis, and identification of AMPK substrate specificity would be useful to understand its role in particular cellular processes and develop strategies to modulate AMPK activity in a substrate-specific manner. Here we show that SUMOylation of AMPKα1 attenuates AMPK activation specifically towards mTORC1 signalling. SUMOylation is also important for rapid inactivation of AMPK, to allow prompt restoration of mTORC1 signalling. PIAS4 and its SUMO E3 ligase activity are specifically required for the AMPKα1 SUMOylation and the inhibition of AMPKα1 activity towards mTORC1 signalling. The activity of a SUMOylati... More