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Gene Synthesis> | ... The coding region derived from A. cepa UGT73G1 mRNA (AY262062), with codons optimized for E. coli heterologous expression, was synthesized and cloned into the E. coli expression vector pET-28a (+) (Novagen) by GenScript (Nanjing, China). ... | Get A Quote |
Glycosylation of quercetin using flavonol-specific glycosyltransferases offers an alternate method for isoquercitrin production. Obtaining sufficient quantities of bioactive enzymes is an important prerequisite for highly effective biocatalysis and biotransformation. In this study, a codon-optimized gene for the flavonoid glucosyltransferase UGT73G1 from Allium cepa was heterologously expressed in the preferred prokaryotic expression host Escherichia coli. By combining expression as a fusion protein with 6-histidine tags with coexpression with molecular chaperones, increased soluble expression of UGT73G1 was achieved in E. coli. Two-terminal 6-histidine tags contributed more to the expression than molecular cha... More