Intelectin mediated phagocytosis and killing activity of macrophages in blunt snout bream (Megalobrama amblycephala).

Intelectin， a lectin discovered recently， has been identified in various vertebrate species， such as fish， amphibians， and mammals. In one of our previous studies， the efficient bacteria binding and agglutinating activity of the recombinant Megalobrama amblycephala intelectin protein (rMamINTL) and the enhanced immunopositive localization have been observed in the hepatic macrophage-like cells (kupffer cells) post Aeromonas hydrophila infection. Thus， the present study primarily focuses on the regulatory effects of rMamINTL on M. amblycephala macrophages. This study revealed a prominent LPS-binding activity of rMamINTL and a significantly increased phagocytosis of rMamINTL-treated A. hydrophila by M. amblycephala macrophages. However， the rMamINTL-treated M. amblycephala macrophages exhibited no evident regulatory effect on phagocytosis， whereas the enhanced killing activity of the rMamINTL-treated macrophages was observed， which may be attributed to the induced respiratory burst activity and the expression of inflammatory cytokines. In addition， the anti-proliferation effect of rMamINTL on two tumor cells was observed. However， its mechanism remains to be further studied. In short， these results show that MamINTL is a multifunctional immune protein with effective immunomodulatory activity.