Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | Human Adra2A was from Genscript (Piscataway,NJ),Gai2 mutantsfromcDNAResourceCenter;TRPC1,TRPC3,andTRPC6wereclonedintopcDNA3intheJuliuslab;TRPC4 was a gift from J. Jeon and M. Zhu; Olfr558 constructs were gifts from H. Matsunami (Addgene) and were tagged with the first 20 residues of human rhodopsin to increase expression; RTP1S, Ric8b, and Gaolf/15 were gifts from A. Chang. lentiCRISPR v2 was a gift from F. Zhang (Addgene). | Get A Quote |
Dietary, microbial, and inflammatory factors modulate the gut-brain axis and influence physiological processes ranging from metabolism to cognition. The gut epithelium is a principal site for detecting such agents, but precisely how it communicates with neural elements is poorly understood. Serotonergic enterochromaffin (EC) cells are proposed to fulfill this role by acting as chemosensors, but understanding how these rare and unique cell types transduce chemosensory information to the nervous system has been hampered by their paucity and inaccessibility to single-cell measurements. Here, we circumvent this limitation by exploiting cultured intestinal organoids together with single-cell measurements t... More