| Products/Services Used | Details | Operation |
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| Proteins, Expression, Isolation and Analysis | After culturing for four days at 37°C under 5% CO2 in 6-well plates, the supernatants were harvested via centrifugation, and antibodies were purified using Protein A (GenScript, Inc., USA) and quantified using a NanoDrop 2000. | Get A Quote |
Classical swine fever (CSF) is a highly contagious swine disease that causes devastating economic losses. However， there are few efficacious therapeutic antibodies against the CSF virus (CSFV). Accordingly， we isolated two whole-porcine anti-CSFV neutralizing antibodies (NAbs) directly from single B cells sorted using the conserved linear epitope of the CSFV E2 protein and goat anti-pig IgG. These mAbs， termed HK24 and HK44， can bind to the E2 protein by recognizing sites within the conserved linear epitope of E2. In addition， these two mAbs can detect virus infection with high specificity and possess potent neutralizing activity. HK24 and HK44 protect PK-15 cells from CSFV infections in vitro with po... More
