Products/Services Used | Details | Operation |
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Custom Vector Construction> | MlaD DNA corresponding to its periplasmic domain (residues 23–183) was chemically synthesized (GenScript) and cloned into the pET vector pET26b (Merck Millipore) to contain a C-terminal hexa-histidine tag | Get A Quote |
The Mla pathway is believed to be involved in maintaining the asymmetrical Gram-negative outer membrane via retrograde phospholipid transport. The pathway is composed of three components: the outer membrane MlaA-OmpC/F complex, a soluble periplasmic protein, MlaC, and the inner membrane ATPase, MlaFEDB complex. Here, we solve the crystal structure of MlaC in its phospholipid-free closed apo conformation, revealing a pivoting β-sheet mechanism that functions to open and close the phospholipid-binding pocket. Using the apo form of MlaC, we provide evidence that the inner-membrane MlaFEDB machinery exports phospholipids to MlaC in the periplasm. Furthermore, we confirm that the phospholipid export... More