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Proteins, Expression, Isolation and Analysis> | Cartilage biomimetic degradable hydrogels were fabricated via photopolymerization of 9wt% PEG-norbornene (8-arm, 10kDa), 1wt% ChS-SH, 0.1mM CRGDS (Genscript), and 2.5wt% MMP7 sensitive peptide (CRDPLE-LRADRC)(43) (Genscript) in the presence of 0.05wt% photoinitiator Igracure 2959 (I2959) in phosphate buffer saline (PBS) under 352nm light at 5 mW cm-2 for 8 minutes. | Get A Quote |
Cartilage tissue engineering strategies that use in situ forming degradable hydrogels for mesenchymal stem cell (MSC) delivery are promising for treating chondral defects. Hydrogels that recapitulate aspects of the native tissue have the potential to encourage chondrogenesis, permit cellular mediated degradation, and facilitate tissue growth. This study investigated photoclickable poly(ethylene glycol) hydrogels, which were tailored to mimic the cartilage microenvironment by incorporating extracellular matrix analogs, chondroitin sulfate and RGD, and crosslinks sensitive to matrix metalloproteinase 7 (MMP7). Human MSCs were encapsulated in the hydrogel, cultured up to nine weeks, and assessed by m... More