Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis> | . Magnetic beads were pre-incubated with 5 μg of custom-made antibody against A. stephensi AGO1 (GenScript) (Supplementary Fig. 12) or normal mouse IgG (Millipore). | Get A Quote |
Insecticidal fungi represent a promising alternative to chemical pesticides for disease vector control. Here, we show that the pathogenic fungus Beauveria bassiana exports a microRNA-like RNA (bba-milR1) that hijacks the host RNA-interference machinery in mosquito cells by binding to Argonaute 1 (AGO1). bba-milR1 is highly expressed during fungal penetration of the mosquito integument, and suppresses host immunity by silencing expression of the mosquito Toll receptor ligand Spätzle 4 (Spz4). Later, upon entering the hemocoel, bba-milR1 expression is decreased, which avoids induction of the host proteinase CLIPB9 that activates the melanization response. Thus, our results indicate that the pathogen deploys a cr... More