Products/Services Used | Details | Operation |
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Gene Synthesis> | Codon-optimized synthetic genes (GenScript) encoding for GPCLASV (Josiah) and GPCLCMV (Armstrong) were served as templates for PCR to generate GP2LASV and GP2LCMV that were subcloned into the pACgp67b expression vector (BD Biosynthesis) to include an N-terminal 6XHis tag followed by TEV protease cleavage site. | Get A Quote |
Lassa virus (LASV) is a notorious human pathogen in West Africa. Its class I trimeric spike complex displays a distinct architecture, and its cell entry mechanism involves unique attributes not shared by other related viruses. We determined the crystal structure of the GP2 fusion glycoprotein from the spike complex of LASV (GP2) in its post-fusion conformation. GP2 adopts a canonical helical bundle configuration similarly to other viruses in its family. The core packing of GP2, however, is more organized compared to GP2 from other viruses reducing the formation of internal hydrophobic cavities. We demonstrate a link between the formation of such unfavorable hydrophobic cavities and the efficiencies of mem... More