Products/Services Used | Details | Operation |
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Gene Synthesis> | The mutated hilD gene with native promoter (318 bp upstream from the translation start site) and C‐terminal 3X‐FLAG tag (Uzzau et al., 2001) was synthesized by GenScript (Piscataway, NJ) (full sequence provided in Supporting Information Fig. 4) and delivered on a pUC57Kan vector, into which we cloned the chloramphenicol acetyl transferase gene from pKD3 (Datsenko and Wanner, 2000) to allow for subsequent selection. | Get A Quote |
N-Lysine acylation is a post-translational modification important for both prokaryotic and eukaryotic cells to control a wide array of cellular functions. Here we demonstrate that the protein acyltransferase Pat regulates genes on Salmonella Pathogenicity Island 1 (SPI1) that are required for the invasion of the intestinal epithelium. Mutation of pat slightly increased spleen colonization by Salmonella in streptomycin-treated mice, with more of the pat mutant reaching the spleen than the wild type strain. Growth of Salmonella under specific conditions selectively induced expression of Pat, and deletion of pat increased SPI1 gene expression under the same growth conditions. In addition, over-expression of ... More