资源 » 参考数据库 » 客户发表文献

至今，GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次，处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果，再次证明GenScript 有能力帮助业内科学家Make research easy.

Tankyrase Sterile α Motif Domain Polymerization Is Required for Its Role in Wnt Signaling.

Structure. 2016;

Riccio AA, McCauley M, Langelier MF, Pascal JM.

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	EXPERIMENTAL PROCEDURES Gene Cloning and Mutagenesis A synthetic gene (GenScript) coding for the human TNKS1 SAM domain (res- idues 1,026–1,092) was cloned into the BamHI/XhoI restriction sites of a pET28 vector (Novagen) modiﬁed to include an N-terminal His6 and SUMO-like tag.... Gene mutations (G1074D, V1056H/R, and V1056H/G1074D) and truncations (DHPS, deletion of 1–173; DCAT, deletion of 1,093–1,327; HPS/ankyrin repeats, deletion of 962–1,327; DSAM, deletion of 1,026–1,092) were performed using the QuikChange Mutagenesis Kit (Stratagene) and veriﬁed by automated DNA sequencing (GenScript, Sidney Kimmel Cancer Center, or Institut de Recherche en Immunologie et en Cance´ rologie).	Get A Quote

摘要

Tankyrase-1 (TNKS1/PARP-5a) is a poly(ADP-ribose) polymerase (PARP) enzyme that regulates multiple cellular processes creating a poly(ADP-ribose) posttranslational modification that can lead to target protein turnover. TNKS1 thereby controls protein levels of key components of signaling pathways, including Axin1, the limiting component of the destruction complex in canonical Wnt signaling that degrades β-catenin to prevent its coactivator function in gene expression. There are limited molecular level insights into TNKS1 regulation in cell signaling pathways. TNKS1 has a sterile α motif (SAM) domain that is known to mediate polymerization, but the functional requirement for SAM polymerization has not been asse... More