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Plasmid DNA Preparation> | Circular dichroism spectra analysis and isothermal titration calorimetry A truncated version of chicken MDM2 (NCBI accession number NP_001186313) containing amino acid residues 204–298 (MDM2204-298) was synthesized (GenScript) and cloned into the expression vector pSY5, a modified pET-21d(+) plasmid (Novagen), encoding an 8-histidine tag ahead of the N-terminus of the protein [40]. | Get A Quote |
Sex-linked barring is a fascinating plumage pattern in chickens recently shown to be associated with two non-coding and two missense mutations affecting the ARF transcript at the CDKN2A tumor suppressor locus. It however remained a mystery whether all four mutations are indeed causative and how they contribute to the barring phenotype. Here, we show that Sex-linked barring is genetically heterogeneous, and that the mutations form three functionally different variant alleles. The B0 allele carries only the two non-coding changes and is associated with the most dilute barring pattern, whereas the B1 and B2 alleles carry both the two non-coding changes and one each of the two missense mutations causing the Sex-lin... More