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ORF cDNA Clones/MolecularCloud> | … Subcellular localization of ORF3 was first predicted in silico by WoLFPSORT program (https://www.genscript.com/wolf-psort.html), and results revealed that the protein has 12–17% identity with the integral membrane proteins such as V-type proton ATPase 16 kDa proteolipid … | Get A Quote |
The porcine epidemic diarrhea virus (PEDV) is an important swine pathogen responsible for severe watery diarrhea, particularly in neonatal piglets. Despite extensive studies performed to elucidate the function of several viral proteins, the contribution of an accessory protein ORF3 in PEDV replication is still largely unknown. Here, we constructed expression plasmids as well as recombinant PEDV carrying myc-tagged ORF3 to assess their expression and subcellular localization in both transfected and infected cells. In PEDV-infected cells, ORF3 was predominantly localized in the cytoplasm, partially in the endoplasmic reticulum (ER) and the Golgi apparatus (Golgi). Interestingly, ORF3 with the N-terminal Flag tag ... More