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Gene Synthesis> | … The NDK gene of Acinetobacter baumannii (strain SDF) was commercially synthesized (Genscript). Using polymerase chain reaction (PCR), the gene was amplified and cloned into a pET-28a vector (Novagen) with an N-terminal hexa-histidine tag … | Get A Quote |
Acinetobacter baumannii is a new threat in intensive care units (ICUs) for its multiresistance to antibiotics, but little is known about this bacterium. Nucleoside diphosphate kinase (NDK) is an evolutionarily conserved enzyme that catalyzes phosphoryl transformation between nucleosides. In our study, the crystal structure of wild type Acinetobacter baumannii NDK along with its mutant generated through truncation of the C-terminal arginine-threonine-arginine (RTR) residues, were solved. In comparison with Myxococcus xanthus NDK structure, we speculated that Acinetobacter baumannii NDK shared a similar catalytic mechanism with Myxococcus xanthus. Activity assay and CD spectra analysis revealed that E28A mutant m... More