Products/Services Used | Details | Operation |
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Gene Synthesis> | To construct an affibody and scFv fusion protein with specificities to both the extracellular domain of HER2 and the hapten, a DNA plasmid of the monomeric HER2 affibody (ZHER2:477) with the His6-tag at the C-terminus and linker (G4S)3 was first custom synthesized (GenScript). | Get A Quote |
In this study, an anti-hapten antibody (single chain Fv, scFv) against a hapten probe was developed as a unique reporter system for molecular imaging or therapy. The hapten peptide (histamine-succinyl-GSYK, Him) was synthesized for phage displayed scFv affinity selection and for conjugation with cypate (Cy-Him) for in vivo near-infrared (NIR) optical imaging. Hapten-specific scFvs were affinity selected from the human single fold phage display scFv libraries (Tomlinson I + J) with high specificity and affinity. Utilizing HER2 targeting as a model system, the highest affinity scFv (clone J42) was recombinantly fused to an anti-HER2 affibody (scFv-L-Aff) with no loss of affinity of either protein. The functionali... More