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Molecular Biology Reagents> | For each of the six CVN2L0 variants (Fig. S14, ESI†) and CVN2L0 wild-type protein with an N-terminal pelB leader sequence and His-tag, lyophilized plasmid DNA was obtained from GenScript® (pET-27b(+) vector; Piscataway, USA) and dissolved in sterile ddH2O to a final concentration of 100 ng μL−1. | Get A Quote |
Cyanovirin-N (CV-N) has been shown to reveal broad neutralizing activity against human immunodeficiency virus (HIV) and to specifically bind Manα(1→2)Manα units exposed on various glycoproteins of enveloped viruses, such as influenza hemagglutinin (HA) and Ebola glycoprotein. Chemically synthesized dimannosylated HA peptides bound domain-swapped and dimeric CV-N with either four disulfide-bonds (Cys–Cys), or three Cys–Cys bonds and an intact fold of the high-affinity binding site at an equilibrium dissociation constant KD of 10 μM. Cys–Cys mutagenesis with ion-pairing amino-acids glutamic acid and arginine was calculated by in silico structure-based protein design and allowed for recognizing dima... More