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Proteins, Expression, Isolation and Analysis> | … (50 mmol/L Tris, 8 mol/L urea, pH 80) The resulting solution was centrifuged, and the supernatant was purified by using a Ni-NTA column The purified protein was sent to GenScript (China, Nanjing) to prepare PaVTG-specific antibodies 26 Western-blotting analysis … | Get A Quote |
In order to investigate the endocrine toxicity of B(a)p to marine polychaete Perinereis aibuhitensis, vitellogenin (VTG) cDNA from the P. aibuhitensis was isolated, recombinated and expressed for the first time. The full length P. aibuhitensis vitellogenin gene (PaVTG) was 5 325 bp, and encoded 1 692 amino acids. It contained the vitellogenin_N domain of unknown function (DUF1943), a von Willebrand factor type D domain, as well as a conserved KALGNAG motif. The expression of VTG gene and protein were mainly up-regulated after exposed to B(a)p at transcriptional and translational levels. PaVTG gene expression did not change significantly at day 4. At day 7 PaVTG expression was up-regulated in 0.5 μg/L and 5 μg... More