Products/Services Used | Details | Operation |
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DNA Sequencing> | … (Nanjing, China). Relevant molecular reagents kits including bacteria genomic DNA kit, plasmid miniprep kit, gel mini purification kit and SDS-PAGE kit were purchased from Zoman Biotechnology, Co. (Beijing, China) … Sequencing service was supplied by GenScript Biotech Co … | Get A Quote |
A new protein immobilization and purification system has been developed based on the improved plasmid vectors, designated pETChBD-X, which contained the gene coding for two novel chitin-binding domains ChBD-AB, factor Xa cleavage site and adapted for gene fusions. The ChBD-AD from SYBC-H1 was used as a novel affinity tag to anchor fusion proteins to chitin granules. The granules carrying the ChBD-AD fusion proteins can be isolated by a simple centrifugation step and used directly for some applications. Moreover, when required, a practically pure preparation of the soluble recombination protein can be obtained after Factor Xa cleavage. The efficiency of this system has been demonstrated by reaching 95% of prote... More