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Gene Synthesis> | Synthetic genes optimized for expression in E. coli (Genscript, Piscataway, NJ, USA) were cloned in frame with a C-terminal 6xHis-Tag into pET-21a vector (EMD, Millipore, Billerica, MA, USA) between NdeI and XhoI sites to obtain, pET-21a [Ef-SOD1a] | Get A Quote |
Bacterial Expression System> | Get A Quote |
Oxidative stress is a particularly severe threat to Antarctic marine polar organisms because they are exposed to high dissolved oxygen and to intense UV radiation. This paper reports the features of three superoxide dismutases from the Antarctic psychrophilic ciliate Euplotes focardii that faces two environmental challenges, oxidative stress and low temperature. Two out of these are Cu,Zn superoxide dismutases (named Ef-SOD1a and Ef-SOD1b) and one belongs to the Mn-containing group (Ef-SOD2). Ef-SOD1s and Ef-SOD2 differ in their evolutionary history, expression and overall structural features. Ef-SOD1 genes are expressed at different levels, with Ef-SOD1b mRNA 20-fold higher at the ciliate optimal temperature o... More