Products/Services Used | Details | Operation |
---|---|---|
Mutant Libraries> | … Helraiser transposase (HR) and mutants were prepared and expressed by GenScript (Piscataway, NJ) in SF9 cells and purified as described previously (Grabundzija et al., 2016). Briefly, N-terminally His-tagged HR was solubilized by sonication and purified on a HiTrap … | Get A Quote |
Helitrons are widespread eukaryotic DNA transposons that have significantly contributed to genome variability and evolution, in part because of their distinctive, replicative rolling-circle mechanism, which often mobilizes adjacent genes. Although most eukaryotic transposases form oligomers and use RNase H-like domains to break and rejoin double-stranded DNA (dsDNA), Helitron transposases contain a single-stranded DNA (ssDNA)-specific HUH endonuclease domain. Here, we report the cryo-electron microscopy structure of a Helitron transposase bound to the 5'-transposon end, providing insight into its multidomain architecture and function. The monomeric transposase forms a tightly packed assembly that buries the cov... More