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Proteins, Expression, Isolation and Analysis> | … The GST-Zfhx4 fusion protein was purified by the GST Fusion Protein Purification Kit (Genscript) according to the manufacture’s protocol. An immunizing emulsion was prepared by mixing the purified peptide (1 mg/ml in PBS, pH 7.5) with an equal volume of complete or … | Get A Quote |
Breathing is an integrated motor behavior that is driven and controlled by a network of brainstem neurons. Zfhx4 is a zinc finger transcription factor and our results showed that it was specifically expressed in several regions of the mouse brainstem. Mice lacking Zfhx4 died shortly after birth from an apparent inability to initiate respiration. We also found that the electrical rhythm of brainstem‒spinal cord preparations was significantly depressed in Zfhx4-null mice compared to wild-type mice. Immunofluorescence staining revealed that Zfhx4 was coexpressed with Phox2b and Math1 in the brainstem and that Zfhx4 ablation greatly decreased the expression of these proteins, especially in the retrotrapezoid nucl... More