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Custom Vector Construction> | … It was not until 2010, when a new highly virulent strain of PEDV emerged in China, that cases began to be seen throughout the … 1a, consisting of residues 1616–1945 with an engineered 6ÂHis tag, was cloned into the pET-15b expres- sion vector (GenScript) and transformed … | Get A Quote |
Porcine epidemic diarrhea is a devastating porcine disease that is caused by the alphacoronavirus porcine epidemic diarrhea virus (PEDV). Like other members of the Coronaviridae family, PEDV encodes a multifunctional papain-like protease 2 (PLP2) that has the ability to process the coronavirus viral polyprotein to aid in RNA replication and antagonize the host innate immune response through cleavage of the regulatory proteins ubiquitin (Ub) and/or interferon-stimulated gene product 15 (ISG15) (deubiquitination and deISGylation, respectively). Because Betacoronavirus PLPs have been well characterized, it was sought to determine how PLP2 from the alphacoronavirus PEDV differentiates itself from its related counte... More