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Plasmid DNA Preparation> | … Plasmid encoding His-tagged full-length ALDH1A1, 1A2, 1A3, and 3A1 were customsynthesized by GenScript. The plasmids were each transformed into BL21(DE3) cells, and each … | Get A Quote |
The identification of biologically active target compounds and their binding proteins is important in mechanism-of-action studies for drug development. Additionally, the newly discovered binding proteins provide unforeseen ideas for novel drug discovery and for subsequent structural transformation to improve target specificity. Based on the lead and final candidate compounds related to the type 5 phosphodiesterase (PDE5) inhibitor E4021, we designed chemical probes and identified their target proteins by the affinity chromatography approach. Aldehyde dehydrogenase family 1 member A3 (ALDH1A3), currently reported as a cancer stem cell target, was clearly isolated as a binding protein of the lead "immature" inhib... More