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Role of Small Envelope Protein in Sustaining the Intracellular and Extracellular Levels of Hepatitis B Virus Large and Middle Envelope Proteins

Viruses. 2021-04; 
Jing Zhang, Yongxiang Wang, Shuwen Fu, Quan Yuan, Qianru Wang, Ningshao Xia, Yumei Wen, Jisu Li, Shuping Tong
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Proteins, Expression, Isolation and Analysis Following transfer, the Western blots were incubated at 4 °C overnight with mouse monoclonal anti-preS1 (7H11; 1:4000), rabbit polyclonal anti-preS2 (GenScript, Shanghai, China; 1:2000) Get A Quote

摘要

Hepatitis B virus (HBV) expresses co-terminal large (L), middle (M), and small (S) envelope proteins. S protein drives virion and subviral particle secretion, whereas L protein inhibits subviral particle secretion but coordinates virion morphogenesis. We previously found that preventing S protein expression from a subgenomic construct eliminated M protein. The present study further examined impact of S protein on L and M proteins. Mutations were introduced to subgenomic construct of genotype A or 1.1 mer replication construct of genotype A or D, and viral proteins were analyzed from transfected Huh7 cells. Mutating S gene ATG to prevent expression of full-length S protein eliminated M protein, reduced intracell... More

关键词

core protein, envelope proteins, hepadnaviridae, hepatitis B surface antigen, hepatitis B virus, secretion, subviral particles, virions, virus evolution