Products/Services Used | Details | Operation |
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Synthetic sgRNA and crRNA Service> | Recombinant plasmids harboring MtbRimM gene (pET-22b, with a C-terminal His6-tag) or MtbS19 gene (pET-28a, with thrombin-cleavable N-terminal His6-tag) were commercially synthesized (GenScript, Nanjing, China) Boundaries for the N-terminal domain | Get A Quote |
Multidrug-resistant tuberculosis (TB) is a serious threat to public health, calling for the development of new anti-TB drugs. Chaperon protein RimM, involved in the assembly of ribosomal protein S19 into 30S ribosomal subunit during ribosome maturation, is a potential drug target for TB treatment. The C-terminal domain (CTD) of RimM is primarily responsible for binding S19. However, both the CTD structure of RimM from (RimM) and the molecular mechanisms underlying RimM binding S19 remain elusive. Here, we report the solution structure, dynamics features of RimM, and its interaction with S19. RimM has a rigid hydrophobic core comprised of a relatively conservative six-strand β-barrel, tailed with a short α-he... More