Products/Services Used | Details | Operation |
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Mammalian Expression> | JPH3 and JPH4 The cDNAs that encode human JPH3 and JPH4, with the eight-residue FLAG sequence linked to the C-terminus, were obtained from GenScript | Get A Quote |
Junctions between the endoplasmic reticulum and plasma membrane that are induced by the neuronal junctophilins are of demonstrated importance, but their molecular architecture is still poorly understood and challenging to address in neurons. This is due to the small size of the junctions and the multiple isoforms of candidate junctional proteins in different brain areas. Using colocalization of tagged proteins expressed in tsA201 cells, and electrophysiology, we compared the interactions of JPH3 and JPH4 with different calcium channels. We found that JPH3 and JPH4 caused junctional accumulation of all the tested high-voltage-activated Ca isoforms, but not a low-voltage-activated Ca. Also, JPH3 and JPH4 noticeab... More