| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … PBS, permeabilized with 0.2% Triton X-100 in PBS for 5 min at room temperature, blocked with 5% BSA in PBS for 30 min at 37 C, and incubated in rabbit anti-VHH primary (GenScript) … | Get A Quote |
Lipid nanoparticles (LNPs) are a clinically relevant way to deliver therapeutic mRNA to hepatocytes in patients. However, LNP-mRNA delivery to end-stage solid tumors such as head and neck squamous cell carcinoma (HNSCC) remains more challenging. While scientists have used in vitro assays to evaluate potential nanoparticles for HNSCC delivery, high-throughput delivery assays performed directly in vivo have not been reported. Here we use a high-throughput LNP assay to evaluate how 94 chemically distinct nanoparticles delivered nucleic acids to HNSCC solid tumors in vivo. DNA barcodes were used to identify LNP, a novel LNP for systemic delivery to HNSCC solid tumors. Importantly, LNP retains tropism to HNSCC solid... More
