GenScript’s offerings are cost-effective with reliable and proven performance for removal of contaminating nucleic acids. Furthermore, the Benz-Neburase’s offerings are produced under stringent quality controls with materials and manufacturing process that are fully traceable, allowing for support of IND filing and progression of development stages for various applications.
alternative to Benzonase®Benzonase
is a registered trademark of Merck KGaA
描述
The Benz-Neburase™ is an endonuclease capable of removing all forms of DNA and RNA, including double stranded, single stranded, linearized, and circular forms.
Benz-Neburase™ GMP, tag free (Cat. No. Z03708) is manufactured in compliance with ISO 9001 and ISO 13485 quality management system standards and with more stringent process controls and relatively complete document records.
GenScript Benz-Neburase™ Nuclease ELISA Kit is a sensitive assay to detect and quantify residual endonuclease impurity in purification and manufacturing processes of viral vectors and vaccines where utilized Benz-Neburase™ Nuclease or other Serratia marcescens endonucleases. Benz-Neburase™ Nuclease ELISA Kit is based on sandwich ELISA with a detection limit of 8.40 pg/ml. It is a simple, accurate and reproducible solution for residual endonuclease quantitation to add to the purification process, quality control, and release testing. Benz-NeburaseTM Nuclease is genetically engineered endonuclease from S. marcescens that cleaves all forms of DNA and RNA non-specifically, including single stranded, double stranded, linear and circular. They are mostly used to remove residual nucleic acids during production process of biological molecules in biopharmaceutical manufacturing including but not limited to vaccine production, viral vector production, and manufacturing of gene and cell therapy-related products.
Deoxyribonuclease I (DNase I) is DNA-specific endonuclease that cleaves both single-stranded DNA,double-stranded DNA and DNA-RNA hybrids, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3' producing tetranucleotides. The activity of DNase I is strictly dependent on Ca2+ and can be activated by divalent metal ions such as Mg2+ or Mn2+. In the presence of Mg2+, DNase I nonspecifically recognizes and cleaves a double-stranded DNA at anysite on either strand, and in the presence of Mn2+, it recognizes and cleaves almost the same sites on both strands of the DNA to produce DNA fragments with blunt ends or sticky ends with 1~2 nucleotide overhangs. GenScript is offering DNase I produced by expression in a P. pastoris strain carrying a plasmid encoding the bovine DNase I.