Removal of plasmid and host residual DNA during Lentivirus (LV) production

Step 1: Dilute Benz-Neburase to 10 kU/ml and place it in a chromatography refrigerator at 4 °C for later use.
Step 2: Mix the harvested cell suspension (5 ml) and add 10 μl Benz-Neburase, mix thoroughly and place in a 37 °C water bath for 60 min.
Step 3: After the incubation, centrifuge at 1300 g for 10 min to remove the cells and cell debris.
Step 4: After the centrifugation, measure the HCD, plasma residues in the samples, and Fluorescence activated Cell Sorting (FACS) can be used to determine the functional titer of lentivirus particles.

The test results show that Benz-Neburase can remove DNA and plasmid residue in virus production process more effectively than the competitor product. The use of Benz-Neburase has minimal impact on the viral production recovery. The recovery data further indicates that the GenScript Benz-Neburase outperforms the competitor product.

Removal of plasmid and host residual DNA during adeno associated virus (AAV) production

Step 1: After harvesting cell suspension, break up the cells, then add 100 U Benz-Neburase to 2 ml cell suspension, mix thoroughly and place in a 37°C water bath for 60 min.
Step 2: After the incubation, centrifuge to remove the cells and cell debris at 1600 g for 10 min.
Step 3: After the centrifugation, measure the HCD and plasmid DNA residues in the samples, and Viral genome (Vg) recovery was used to quantify the adeno associated viral production.

The test results show that Benz-Neburase can also remove DNA and plasmid residue in AAV virus production process more effectively than the competitor product. The use of Benz-Neburase has minimal impact on the viral production. The data further indicates that the GenScript Benz-Neburase outperforms the competitor product

Reduction of the viscosity of bacterial lysate

Step 1: Centrifuge the bacterial culture, remove the supernatant, and then add the lysate.
Step 2: Treat the sample with Benz-Neburase at a final concentration of 2.5 U/ml, incubate at 37 °C for 30 min.
Step 3: Centrifuge to observe the viscosity of the precipitate and supernatant.

The test results show that Benz-Neburase can greatly reduce the viscosity of bacterial lysate.

Prevention of cell clumping

Step 1: Spread the adhered cells in a 24-well plate and treat them with control buffer (left) and 50 U/ml Benz-Neburase (right) at 37 °C for 30 min.
Step 2: Observe the cells using a microscope

The test result show that Benz-Neburase can efficiently reduce cell clumping.

Prevention of cell clumping

Step 1: Treat SUP-T1 cells (left) and NK-92 cells (right) with 2 μl of Benz-Neburase at different concentrations (20-500 U/ml).
Step 2: Incubate overnight in an incubator at 37 °C in 5% CO2.

The test results indicate Benz-Neburase has minimal to no impact on the cell viability.

Lane M：DNA marker
Lane 1：PCR Product
Lane 2：Benz-Neburase + PCR Product
Lane 3：Competitor endonuclease + PCR product
Lane 4：Genomic DNA
Lane 5：Benz-Neburase + Genomic DNA
Lane 6：Competitor endonuclease + Genomic DNA
Lane 7：Plasmid DNA
Lane 8：Benz-Neburase + Plasmid DNA
Lane 9：Competitor endonuclease + Plasmid DNA
Lane 10：RNA
Lane 11：Benz-Neburase + RNA
Lane 12：Competitor endonuclease + RNA

In 20 μl reaction volume, use 1U of Benz-Neburase to digest different kinds of nucleic acid at 37 °C for 10 min.

The test results show that Benz-Neburase is effective in digesting various forms of DNA and RNA, with efficiency identical to competing products.