| Reconstitution |
Reconstitute
the lyophilized product with deionized water (or equivalent) to the final
concentration of 1.0 mg/ml. |
| Concentration |
1 mg/ml,
lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide. |
| Storage |
The product
remains stable in lyophilized form for at least 1 year if stored at 2-8°C,
-20°C, or lower from the date of receipt. For longer storage, -20°C or below is
recommended. The reconstituted product can be stored for 2-3 weeks at 2-8°C or
for up to 1 year at -20°C or below. Avoid repeated freezing and thawing cycles. |
|
Working
concentrations for specific applications should be determined by titration
assay. The chart below may serve as a useful guideline, but primary antibody, temperature,
incubation time, the amount of antigen, and the sensitivity of the detection
methods can all affect the ideal working concentration. |
| Application |
Recommended Usage |
| Dot blot |
1:2,000-1:20,000 |
| Western blot |
1:2,000-1:20,000 |
| ELISA |
1:20,000-1:100,000 |
| Other applications |
user-optimized |
|
| Target Background |
Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Biotin-Protein L, which is produced by covalently linking biotin to protein L, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry. |
For research use only.
Not intended for human and animal therapeutic or diagnostic use.
