Products/Services Used | Details | Operation |
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FLAG G1 affinity resin> | ...After the second ultracentrifugation, the resolubilized protein was applied to 500 µL washed a-FLAG G1 affinity resin (<b>GenScript</b>)... | Get A Quote |
The peptidoglycan cell wall is essential for the survival and shape maintenance of bacteria. For decades it was thought that only penicillin-binding proteins (PBPs) effected peptidoglycan synthesis. Recently, it was shown that RodA, a member of the Rod complex involved in side wall peptidoglycan synthesis, acts as a peptidoglycan polymerase. Rod A is absent or dispensable in many bacteria that contain a cell wall; however, all of these bacteria have a RodA homologue, FtsW, which is a core member of the divisome complex that is essential for septal cell wall assembly. FtsW was previously proposed flip the peptidoglycan precursor Lipid II to the peripasm, but we report here that FtsW polymerizes Lipid II. We sho... More