Products/Services Used | Details | Operation |
---|---|---|
PCR Cloning and Subcloning> | ... PAGE) was purchased from Sigma-Aldrich. Cloning and expression vector construction. The synthetic human H1.4 gene was purchased from GenScript and was provided as an insert in the pUC57 vector. The H1.4 insert was ... | Get A Quote |
Linker histones are an abundant and critical component of the eukaryotic chromatin landscape. They play key roles in regulating the higher order structure of chromatin and many genetic processes. Higher eukaryotes possess a number of different linker histone subtypes and new data are consistently emerging that indicate these subtypes are functionally distinct. We were interested in studying one of the most abundant human linker histone subtypes, H1.4. We have produced recombinant full-length H1.4 in Escherichia coli. An N-terminal Glutathione-S-Transferase tag was used to promote soluble expression and was combined with a C-terminal hexahistidine tag to facilitate a simple non-denaturing two-step affinity chrom... More