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Custom Vector Construction> | … Annotated sequences of these base vectors are available in GenBank (GAPTrap-IRESMygro, GAPTrap-IRESMuro, GAPTrap-IRESMeo). The Mygro, Meo, and Muro coding sequences were synthesized to order by GenScript (http://www.genscript.com) | Get A Quote |
The ability to reliably express fluorescent reporters or other genes of interest is important for using human pluripotent stem cells (hPSCs) as a platform for investigating cell fates and gene function. We describe a simple expression system, designated GAPTrap (GT), in which reporter genes, including GFP, mCherry, mTagBFP2, luc2, Gluc, and lacZ are inserted into the GAPDH locus in hPSCs. Independent clones harboring variations of the GT vectors expressed remarkably consistent levels of the reporter gene. Differentiation experiments showed that reporter expression was reliably maintained in hematopoietic cells, cardiac mesoderm, definitive endoderm, and ventral midbrain dopaminergic neuron... More